[Bincika Tasirin]

a Mai sauri, mai sauki, mai hankali da kuma ** idan aka kwatanta da hanyar gwajin enzyme-bound **

a Yellow mold ** Mai sauri gaugeFITLY-FQ, sikelin **, sosai m

a Linear ganowa kewayon:0.5 - 100 ppb

【Tsarin bincike】

Lura:Kafin bincike, a sanya kowane abu da aka yi amfani da bincike zuwa zafin jiki na ɗaki.

Bayani:AflaQuikCikakken kwalliya daga samfurin abinci ta hanyar tsari mai sauki ** da kuma auna yawan kwalliya da sauri ta amfani da fluorescent photometers. Ka yi ruwa cirewa na samfurin ta hanyar chromatography shafi don sticking da yellow mold **. Bayan wanke chromatography ginshika ta amfani da tsaftacewa mai tsaftacewa, wanke da methanol daga yellow mold **. An haɗa ruwan wanke tare da reagents na asali don inganta fluorescence na Yellowmold **, sannan an auna shi ta amfani da fluorescence gauge da aka daidaita ta hanyar Yellowmold ** daidaituwa a cikin akwatin kayan aiki.

1. Cire (duba jadawalin).

2. Binciken launi (duba jadawalin).

3. zai50μLƘara wani micro gilashi bututun wanke. Shiga50μLDilute derivative reagents da kuma hada tare da pipeline3sau. Shirya "Sample" bututun.

4. Standard shirye-shiryen: suction100μL100ppbYellow mold ** misali kayayyaki zuwa wani micro gilashi bututu, shirya "misali" bututu. sucking100μLMethanol a matsayin "blank" bututu.

5. Ka kunna fluorescence gauge. calibration mai karatu,Sanya "blank" bututun zuwa samfurin rack.Latsa "calibrate", "duba", sa'an nan kuma "blank" maɓallin. Mai karatu ya fara ƙididdiga. danna“<- Tsarin ->”Hashe kibiya key har zuwa window nuna "100.000". zai"Standard" bututuSaka a cikin samfurin rack.Danna "Ƙididdiga". Mai karatu yana nuna "An kammala daidaitawa". An daidaita mai karatu. Danna "Koma" button.

6. Sanya "Sample" tube zuwa samfurin rack, sa'an nan kuma bi "Measurement"→" Bincike1”→"Ƙididdiga". Yuexin ** concentration za a nuna a cikin taga.Lissafi da kuma tsabtace da dilution factor na Curcumin **, samun jimlar matattarar Curcumin ** na samfurin abinci. Rubuta bayanai, ko danna "Ajiye" key don adana bayanai don dawo. Latsa "Koma" button sa'an nan kuma "Matsayi" na gaba samfurin.

Lura: Idan "samfurin" mayar da hankali ya wuce iyaka, a rarraba samfurin a cikin methanol da kuma sake ganowa.

[Bayanan samfurin]

•AflaQuikBincika akwatin gwaji: cat#:AflaQuik-25.Isa game da25Binciken biyu. Kwayoyin kwayoyin:（1）AlfaQuikTsarin Chromatography (25ya）， （2）10Abubuwan da aka samo (3miliyarta). （3(Yellow Mould ** daidaitaccen kayayyaki)1ml).

Guje wa tuntuɓar ko numfashi. Dole ne a bi tsarin tsaro na dakin gwaje-gwaje, gami da Dokokin Tsaro da Lafiya na Aiki (OSHA) amincewa karewa madubi, hannu da kuma karewa tufafi.

Jirgin & Storage:Akwatin gwaji ya kamata a yi amfani da jigilar sanyaya. Adadin zafin jiki na binciken4°A. Shelf ne bayan karɓar18watanni.

•100 Mini gilashi bututu:GTB-6x25.

•Yellow mold ** Mai sauri gaugeFITLY-FQ.Reader ya zo tare da daya5Voltage DC ikon adaftar, dayaUSBData layi, aiki manual da kuma data management software disks.

【Abinci Samfurin shirya tsari】

Nau'ikan walnut (walnut, walnut, walnut, almond):

1. Cire

a auna25ggrinding samfurin, ƙara5gphysiological salt ruwa, sanya a cikin mixer.

a da100ml na methanol-Ruwa Haɗuwa (60:40(Haɗuwa.

a High gudun hada na minti daya. Zuba fitarwa a cikin takarda mai tacewa mai nunawa, tattara tacewa tare da mai tsabta.

a zai20An canja mililitar da aka tace fitarwa zuwa mai tsabta bututun gwaji. Amfani20ml na ruwan deionization diluted, haɗuwa daidai. An tace da ruwan da aka cire a cikin mai tsabta bututun gwaji ta hanyar matattarar microfiber na gilashi.

2. Binciken launi

a CireAflaQuikSama da ƙasa cover na chromatography ginshike. Jerk ruwa daga saman chromatography ginshike..

a zai10mililitarAn tace da cinye-cinyeda kimanin kowane second1zuwa2Drops gudun gaba daya ta hanyar chromatography ginshikeHar sai kumfa ta ɓace gaba ɗaya.

a zai10ml na ruwa mai deionization a kimanin kowane dakika2Saurin drop ta hanyar chromatography ginshike, bari shi gudana har sai kumfa gaba daya ya ɓace.

a Amfani2ml na ingantaccen ruwa chromatography-grade methanol ya wanke Yellowmold ** a cikin chromatography shafi zuwa wani tsabtace bututun gwaji. Saurin yana kimanin drop daya a kowane dakika.

Turmeric foda, baƙar ciki:

1. Cire

a auna25ggrinding samfurin, ƙara5gphysiological salt ruwa, sanya a cikin mixer.

a da100ml na methanol-Ruwa Haɗuwa (80:20(Haɗuwa.

a High gudun hada na minti daya. Zuba fitarwa a cikin takarda mai tacewa mai nunawa, tattara tacewa tare da mai tsabta.

a zai5mililitarAn tace shiCanja wurin cirewa a cikin wani tsabtace bututun gwaji. Amfani20mililitar10%Twain20Solution diluted, haɗuwa daidai. An tace da ruwan da aka cire a cikin mai tsabta bututun gwaji ta hanyar matattarar microfiber na gilashi.

2. Binciken launi

a CireAflaQuikSama da ƙasa cover na chromatography ginshike. Jerk ruwa daga saman chromatography ginshike..

a zai4ml da aka tace diluted cire zuwa kimanin kowane second1zuwa2Saurin drop gaba daya ta hanyar chromatography column har sai kumfa gaba daya ya ɓace.

a zai10ml na ruwa mai deionization a kimanin kowane dakika2Saurin drop ta hanyar chromatography ginshike.

a Amfani2ml na ingantaccen ruwa chromatography-grade methanol ya wanke Yellowmold ** a cikin chromatography shafi zuwa wani tsabtace bututun gwaji. Saurin yana kimanin drop daya a kowane dakika.

Chili baraba, ja baraba, paprika

（Chili Red Chili Chili foda)

1. Cire

a auna25ggrinding samfurin, ƙara5gphysiological salt ruwa, sanya a cikin mixer.

a da100ml na methanol-Ruwa Haɗuwa (80:20(Haɗuwa.

a High gudun hada na minti daya. Zuba fitarwa a cikin takarda mai tacewa mai nunawa, tattara tacewa tare da mai tsabta.

a zai10An canja mililitar da aka tace fitarwa zuwa mai tsabta bututun gwaji. Amfani40ml na ruwan deionization diluted, haɗuwa daidai. An tace da ruwan da aka cire a cikin mai tsabta bututun gwaji ta hanyar matattarar microfiber na gilashi.

2. Binciken launi

a CireAflaQuikSama da ƙasa cover na chromatography ginshike. Jerk ruwa daga saman chromatography ginshike..

a zai4ml da aka tace diluted cire zuwa kimanin kowane second1zuwa2Saurin drop gaba daya ta hanyar chromatography column har sai kumfa gaba daya ya ɓace.

a zai10ml na ruwa mai deionization a kimanin kowane dakika2Saurin drop ta hanyar chromatography ginshike, bari shi gudana har sai kumfa gaba daya ya ɓace.

a Amfani2ml na ingantaccen ruwa chromatography-grade methanol ya wanke Yellowmold ** a cikin chromatography shafi zuwa wani tsabtace bututun gwaji. Saurin yana kimanin drop daya a kowane dakika.

Masara, Peanut Sauce:

1. Cire

a auna25ggrinding samfurin, ƙara5gphysiological salt ruwa, sanya a cikin mixer.

a da100ml na methanol-Ruwa Haɗuwa (70:30(Haɗuwa.

a High gudun hada minti biyu. Zuba fitarwa a cikin takarda mai tacewa mai nunawa, tattara tacewa tare da mai tsabta.

a zai15An canja mililitar da aka tace fitarwa zuwa mai tsabta bututun gwaji. Amfani30ml na ruwan deionization diluted, haɗuwa daidai. An tace da ruwan da aka cire a cikin mai tsabta bututun gwaji ta hanyar matattarar microfiber na gilashi.

2. Binciken launi

a CireAflaQuikSama da ƙasa cover na chromatography ginshike. Jerk ruwa daga saman chromatography ginshike..

a zai15ml da aka tace diluted cire zuwa kimanin kowane second1zuwa2Saurin drop gaba daya ta hanyar chromatography column har sai kumfa gaba daya ya ɓace.

a zai10ml na ruwa mai deionization a kimanin kowane dakika2Drop gudun ta hanyar chromatography ginshike,Bari shi tafiHar sai kumfa ta ɓace gaba ɗaya.