A jerin kayayyakin da aka tsara musamman don immune fluorescence amsawa seals, da musamman formula sa fluorescence na launi ba ya raguwa.Yawancin amfani da anti-fluorescence fadewa kwayoyin suna daAF1kumaAF2kumaAF3kumaAF4kumaAF87kumaAF100kumaAF200kumaAF300kumaCFM-1kumaCFM-2Jira. Wasu kayayyakin sun ƙunshiDAPI,Za a iya lined nucleus ko linedDNAgwajin.

Game da anti-fluorescence quencher, kowa uku nau'ikan: phenolic isonic acid propylenen-Propyl gallate (NPG)Amine-irin paraphenylaminep-Phenylenediamine (PPD)Amine irin1,4-Diazabicyclo-octane（DABCO) Triethylene diamine. Asalin ka'idar kashewa na fluorescence shine wannan: yau da kullun narkewar oxygen a cikin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin Lura cewa ƙara anti-quencher a cikin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyinDNAHadarin da aka haɗa da farfajiyar amsawa. Duba fa'idodi da rashin amfani da waɗannan reagents uku:PPDZa a iya amsa tare da dandelion dyes (musammanCy2), don haka irin wannan launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launin launinPPDkwayoyin kwayoyin;NPG, ba mai guba, rayuwa cell fluorescent staining fi so irin wannan kwayar, ammaNPGTsarin apoptosis ya tsoma baki, abokan karatun da ke gudanar da irin wannan binciken sun kula da shi sosai, don kauce wa ƙunshe daNPGkwayoyin kwayoyin;DABCOBa kamarPPDDon haka inganci da kuma low guba, za a iya amfani da shi a rayuwa kwayoyin halitta, amma tare daNPGKamar haka, akwai tsoma baki a cikin tsarin apoptosis.

A yawan kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin kwayoyin Sauki ra'ayi ne:

Mataki na farko, coagulation ko non-coagulation? Gudanar da ajiya da ɗaukar yana da sauƙi, a yi la'akari da raguwar ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙwayoyin ƙ Non-coagulation nau'in da ake buƙatar la'akari da shi ne volatility da gefen leakage na sealant, tabbas bukatar rufe slide (wani lokacin kuma bukatar silicone hatimi matsSpacer), kuma sau da yawa cover slide kuma bukatar a rufe, sau da yawa amfani da launin ƙushi, hakori wax,VALAP (daidai da haɗuwa da Vaseline, lanolin, da paraffin haɗuwa a kan farantin dumama 60 ° C)Pain a kusa da murfin slide, launin ƙushi yana ɗaukar lokaci mai tsawo don bushewa, akwai haɗarin gurɓataccen ruwan tabarau; Wax na hakori a zafin jiki na yau da kullun zai iya warkewa da sauri;VALAP (daidai da haɗuwa da Vaseline, lanolin, da paraffin haɗuwa a kan farantin dumama 60 ° C)Ana amfani da shi sosai a cikin ƙwayoyin halitta masu rai.

Mataki na 2: Ana buƙatar glycerol (mai narkewa) ko non-glycerol (mai narkewa)? Glycerin za a iya narkewa da kowane rabo na ruwa, za a iya narkewa da ethanol, amma ba tare da man fetur ba. Da sanin wannan, zaɓi ya fi sauƙi. Menene tsarin amsawa na combinatorial? Da ruwa, zaɓi glycerol (ruwa narkewa). Bayan tsananin dehydration magani, zaɓi non-glycerol (mai narkewa).

Mataki na uku, refractive rate na kwalliya. Dyeing fina-finai da sealing kwayoyin zabi refractive a1．52zuwa1．54mafi kyau transparency tsakanin; Abubuwan da ba su da launi sun fi kyau a rufe su da kayan rufi tare da babban ko ƙananan ƙididdigar ƙididdiga, don kawar da bambancin ƙididdiga don samun mafi kyawun hoto.

Gabatarwar da ake amfani da film-forming agents:

polyethylene alcoholpoly (vinyl giya): organic polymer abu, ba guba, narkewa a ruwa, sau da yawa amfani da wani membrane agent. Daban-daban lambobi dangane da polymerization da alcoholism, misaliMowiol ® 4-88 daga.PVAHakanan ana iya ƙara wasu abubuwa ko halaye don gyare-gyare, misaliPermafix-1Ya canzaPVA.

Polyethylene Pyrolidone(polyvinyl pyrrolidone)takamaiman sunanPVPYana da wani non-ionic polymer mahada, kuma yawanci amfani da film maker.

Jagorar sayen kayayyaki:

a AF1:Ingantaccen abu shine glycerol,PBSbuffer, anti-fluorescence quencher, sauran abubuwa, da dai sauransu, refractive1.46，PH10hagu da dama, dace da gano tissue yanke, mutuwa kwayoyin halitta da aka nutse a cikin glycerol, FISH (ciki har da CARD-FISH)Wait da cover.

a AF2:Basic abubuwa ne glycerol, anti-fluorescence quencher, sauran abubuwa, da dai sauransu, ba tare da kansa buffer. refractive ƙimar1.47Amfanin samfurin shine babu kansa buffer, wanda zai iya dacewa da kowane sauran buffer.

a AF3:Basic abubuwa nePBSbuffer, anti-fluorescence quencher, sauran abubuwa, refractive1.34Ya dace musamman ga gwajin kwayoyin halitta.

a AFR3:Basic abubuwa nePBSbuffer, sabon nau'in anti-fluorescence quencher (non-amino nonphenol), sauran abubuwa da sauransu

a AF4:Har ila yauGlycerol / n-propyl gallate matsakaicin shigarwaShuttering agent, ƙara anti-fluorescence fadewa ne ba tare da esonic acid propylenen-Propyl gallate (NPG).

a AF87: Musamman wuri ne mai dauke da ba fluorescent submersible man fetur, wanda aka tsara musamman don lura a karkashin babban madubi, dominDAPIDyeing yana da amfani musamman (DAPIAkwai yanayin kwarara a cikin kwayoyin glycerol).

a AF100:Basic abubuwa nePBSbuffer, anti-fluorescent fadewa, sauran abubuwa, da dai sauransu, shi ne wani shirye-shirye shirye-shiryen shirye-shiryen shirye-shiryen shirye-shiryen shirye-shiryen shirye-shiryen shirye-shiryen shirye-shiryen shirye-shiryen shirye-shirye shiryeCFPVOHMatching amfani da mafita. Buƙatar Pre-MixedAF100daCFPVOHMagani, da yawa hada da yawa, bayan hadawa10Amfani a cikin sa'o'i. refractive rate na kwayar1.34-1.35.

a AF200:daAF100Kamar, bambanci ne,AF200ne glycerol,Amino-type anti-fluorescence daukaka, dace da anthrax, Alexa, Hoeches da sauransu.

a AF300:daAF100Kamar, bambanci ne,AF300Yana da glycerol-based, phenolic-type anti-fluorescence daukaka.

a CFMThe refractive rate na jerin tablets ne1.52, daidai da ko kusan daidai da refractive rate da aka yi amfani da ɗaukar kaya da murfin gilashi, wannan yana da taimako ga samun cikakken hoto. Ana amfani da shi a ƙarƙashin microscope mai mayar da hankali tare da laser, ko kuma a maimakon mai mai a lokacin da ake buƙatar mai mai mai da mai.CFM-1dagapHgame da6.5，.CFM-1 Ƙari AntifadentdagaPHkimanin darajar10，CFM-2dagapH nagame da~8.5，CFM-3dagaPHValue kusa da tsaka tsaki.

Bayanan oda: